Research Paper Volume 12, Issue 11 pp 11139—11151

Melatonin regulates mitochondrial dynamics and alleviates neuron damage in prion diseases

Figure 6. Melatonin and mitochondrial dynamic proteins regulate mitochondrial function in PrP106-126-induced prion models. (A, B) Protein levels of DRP1 in mitochondria by Western blotting. (C, D) Protein levels of OPA1 in whole cells by Western blotting. (E) Representative photomicrographs of mitochondria by confocal fluorescence microscopy showing mitochondrial morphology (Original magnification 600×). (FH) Morphometric measurement of mitochondria. (IK) Mitochondrial function - ROS production (J), mitochondrial membrane potential (MMP) (K) and ATP levels. *P < 0.05, **P < 0.01, ***P < 0.001, comparison with PrP106-126 group. All experiments were repeated at least three times.