Figure 2. The glucose-6-phosphate dehydrogenase (G6PD) regulator PLK1 is highly expressed in tracheal transplantation allografts. (A) Schematic depicting the establishment of murine heterotopic trachea transplantation models. (B) Heatmap of relative expression values of 14 genes of interest from the microarray data validation as assessed by qRT-PCR in mouse. (C) Trachea grafts were harvested 28 days after transplantation, and the sections were stained for PLK1. (D, E) PLK1 expression in the allograft group compared with isograft group. (F) Total RNA was isolated from grafts and reverse-transcribed into cDNA. The transcript level of PLK1 relative to GAPDH was measured. * P≤0.05, ** P≤0.01, GAPDH; glyceraldehyde-3-phosphate dehydrogenase, PLK; polo-like kinase.