Figure 4. PLK1 inhibition suppresses TGFβ-mediated differentiation of myofibroblasts. (A) Schematic depicting establishment of the in vitro model of TGFβ-mediated differentiation of myofibroblasts. (B) Total RNA was isolated and reverse transcribed into cDNA. The transcript level of α-SMA in the BI6727 treatment group relative to the vehicle group was measured. (C, D) The expression of α-SMA was measured by FCM. Bar diagram shows MFI analysis of α-SMA expression. (E) The expression of α-SMA was measured by WB and quantified by densitometry. (F) The expression of α-SMA was analyzed by IF. (G) Migration ability of myofibroblasts in the vehicle and BI6727 treatment groups. * P≤0.05; FCM: flow cytometry; IF: immunofluorescence; SMA: smooth muscle actin; WB: western blot.