PLK1 siRNA suppresses differentiation of myofibroblasts in vitro and in vivo. (A) Protein expression of PLK1 following siRNA transfection. (B) The mRNA expression of α-SMA following PLK1 siRNA transfection. (C, D) The expression of α-SMA was measured by FCM; quantification by MFI analysis is shown in the bar diagram. (E) The expression of α-SMA was analyzed by IF. (F, G) The expression of α-SMA was analyzed by WB. (H, I) Representative immunoblots for MEK, phosphorylated ERK, and GAPDH at various timepoints are shown. (J) Representative immunohistochemical stains of PLK1 siRNA-treated or control tracheal grafts 28 days post-transplantation. (K) Percentage of luminal occlusion in the PLK1 siRNA-treated and control groups 28 days after transplantation.