Research Paper Volume 12, Issue 12 pp 11653—11666

Long non-coding RNA RP11-59H7.3 promotes cell proliferation and invasion metastasis in colorectal cancer by miR-139-5p/NOTCH1 axis

Figure 3. RP11-59H7.3 promotes proliferation, cycle progression and inhibits apoptosis of CRC cells in vitro. (A) effects of overexpression and knockdown of RP11-59H7.3 on the formation of colonies in colorectal cancer cells. (B) the Edu proliferation assays were applied to evaluate the CRC cell viability prior transfected with pcDNA-RP11-59H7.3 compared to vector control. (C) cell cycle analysis was conducted in both LoVo and SW480 cells prior transfected with sh-1 and sh-NC or vector and pcDNA-RP11-59H7.3. (D) programmed cell death analysis was conducted in sh-NC, sh-1, and sh-2 transfected cell lines. (E) western blot was conducted to analyze the apoptosis-related proteins expression in colorectal cancer cells. ***p < 0.001; ** p < 0.01; * p < 0.05.