Research Paper Volume 12, Issue 12 pp 11685—11697

Long noncoding RNA KCNQ1OT1 promotes colorectal carcinogenesis by enhancing aerobic glycolysis via hexokinase-2

Figure 5. LncRNA KCNQ1OT1 promotes aerobic glycolysis and CRC cell proliferation via HK2. (A) Representative western blot images show HK2 protein levels in parental HCT116 cells (control) and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (B, C) ECAR assay results show extracellular acidification rate (ECAR) in parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. Each data point represents means ± SD. The experiment was repeated three times independently. (D, E) Lactate assay results show lactate levels in parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (F, G) Glucose secretion assay results show the concentration of glucose in the media of parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. (H, I) CCK-8 assay results show proliferation status of parental HCT116 cells and HK2-overexpressing plus KCNQ1OT1-knockdown HCT116 cells. Note: **p < 0.01, ***p < 0.001, NS, not significant.