Research Paper Volume 12, Issue 11 pp 10162—10179

First-in-class candidate therapeutics that target mitochondria and effectively prevent cancer cell metastasis: mitoriboscins and TPP compounds

Figure 6. Mitochondrial inhibitor compounds selectively target and prevent cancer metastasis. MDA-MB-231 cells and the well-established chorio-allantoic membrane (CAM) assay in chicken eggs were used to quantitatively measure spontaneous tumor mestastasis. An inoculum of 1 X 106 MDA-MB-231 cells was added onto the CAM of each egg (on day E9) and then eggs were then randomized into groups. On day E10, tumors were detectable and they were then treated daily for 8 days with vehicle alone (1% DMSO in PBS) or the four mitochondrial inhibitors. After 8 days of drug administration, the lower CAM was collected to evaluate the number of metastatic cells, as analyzed by qPCR with specific primers for Human Alu sequences. Note that all four mitochondrial inhibitors did show significant effects on MDA-MB-231 metastasis. More specifically, all three Mitoriboscins were clearly effective in inhibiting metastasis, although 24/D4 and 24/F9 were the most effective. In addition, Bis-TPP also significantly prevented metastasis. Averages are shown + SEM. *p<0.05; ***p<0.001.