Research Paper Volume 12, Issue 12 pp 11698—11716

Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway

Figure 1. Epothilone B dominantly induced osteoclast apoptosis without cytotoxicity. (A) Chemical structure of Epothilone B. (B–C) CCK8 analysis of cell viability of RAW264.7 cells treated with different concentrations of Epothilone B (0μM to 15μM containing 0.1% DMSO) for 24 h or 72 h. (D–E) Flow cytometry analysis of osteoclast apoptosis during osteoclastogenesis in presence or absence of various dosage of Epothilone B (0μM, 5μM and 10μM). (F–G) Flow cytometry analysis of cell cycle during osteoclast differentiation by administration with or without Epothilone B (5μM and 10μM). Data in the figures represent mean ± SD. N.S. represented no significant difference. *p < 0.05, **p < 0.01, ***p < 0.001 based on one way ANOVA.