Research Paper Volume 12, Issue 12 pp 11698—11716

Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway

Figure 7. Epothilone B prevented LPS-induced inflammatory osteolysis in vivo. (A) Representative μCT longitudinal section images of the femurs, cross-sectional view of the distal femurs and reconstructed trabecular structure of the ROI (red dashed box). (BE) Quantitative μCT analysis of distal femoral volumetric bone mineral density (BMD), BV/TV, trabecular thickness (Tb.Th) and trabecular number (Tb.N) in each group. (F) Representative images of histological slides of HE stain, Masson stain and immunohistological stain for TRAP focusing on the metaphyseal region of the distal femur from mice of different groups. Scale bar = 800 μm (G) Quantitative analysis of the percentage of bone area in each slice which was reflected by HE staining. (H) Quantitative analysis of the number of TRAP positive cells in each slice which was reflected by immunohistological stain for TRAP. Data in the figures represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 based on one way ANOVA.