Research Paper Volume 12, Issue 12 pp 11717—11731

Resveratrol inhibits the malignant progression of hepatocellular carcinoma via MARCH1-induced regulation of PTEN/AKT signaling

Figure 4. Resveratrol could down-regulate MARCH1 expression via PTEN–STAT3 signaling. (A, B) HepG2 and Hep3B cells were treated with different concentrations of resveratrol for 48 h, and the level of protein expression was analyzed by western blotting. MARCH1 and p-AKT levels significantly drastically decreased, PTEN levels increased, and downstream protein molecules also significantly decreased. (C, D) HepG2 cells were treated with inhibitors MK2206 and BPV(phen). The combination of resveratrol and inhibitors significantly inhibited cell survival compared to resveratrol alone. (E) Overexpression of the MARCH1 protein with empty vectors and overexpression plasmids in the human HL7702 cells. (F) HepG2 cells were infected with indicated concentrations of siRNA for 72 h. MARCH1 expression significantly decreased, while PTEN expression increased. (G, H) HepG2 cells were pretreated with an inhibitor for 12 h and then combined with resveratrol for 48 h. MARCH1 expression decreased even more compared to resveratrol alone. GAPDH was also detected as a loading control.