Research Paper Volume 12, Issue 12 pp 11717—11731

Resveratrol inhibits the malignant progression of hepatocellular carcinoma via MARCH1-induced regulation of PTEN/AKT signaling

Figure 5. Resveratrol inhibits tumor growth in HepG2 mouse models. (AC) HepG2 cells were inoculated into the back of BALB/c nude mice, near the right hind leg. The mice were treated with resveratrol (0, 50, and 100 mg/kg/ig). Representative images of the tumor are shown, and the tumor volume growth curves of resveratrol are plotted. (D) Tumor weight after resveratrol treatment. (E) Representative images of mice MRI. (F) Average volume of tumor measured on coronal T2-weighted MRI after resveratrol treatment. (G) The changes in animal weight are shown during resveratrol treatment. (H) Tumor tissue slides were stained with H&E and IHC performed. (I) Levels of MARCH1, PTEN, p-AKT, p-STAT3, VEGF, and Bcl2 in tumors treated with resveratrol were analyzed by western blotting. GAPDH was also detected as a loading control. All values represent the mean ± SD. **P < 0.01; *P < 0.05.