Figure 1. The expression levels of chemerin, and CMKLR1 in different degenerated human NP and blood tissues. (A) Representative MRI images of three different degrees of IDD patients. (B) The expression of levels of chemerin, and CMKLR1 from NPCs of different degrees of IDD patients were analyzed by western blotting. (C, D) Quantification of chemerin, and CMKLR1 immunoblots. (E, F) RT-PCR showed that the mRNA levels of chemerin, and CMKLR1 in the NP tissue were significantly higher compared with AF tissue. Data are represented as mean ± SEM of three independent experiments, each done in triplicate. Significant differences between groups are indicated as **p < 0.01, *p < 0.05.