Research Paper Volume 12, Issue 12 pp 11732—11753

Chemerin facilitates intervertebral disc degeneration via TLR4 and CMKLR1 and activation of NF-kB signaling pathway

Figure 2. Effects of chemerin on the viability, and inflammatory responses of human NPCs Human NPCs were treated with chemerin in a dose dependent for 12 or 24 hours. (A, B) Cell viability of NPCs were determined by Cell Counting Kit-8 (CCK-8). (C) The expression levels of COX-2, TNF-α, IL-1β, and IL-6 were visualized by western blotting. (DF, H) Quantification of COX-2, TNF-α, IL-1β, and IL-6 immunoblots in NPCs. (G) The mRNA expression levels of iNOS, COX-2, IL-1β, IL-6, and TNF-α in NPCs were evaluated by RT-PCR. Data are represented as mean ± SEM of three independent experiments, each done in triplicate. Significant differences between groups are indicated as **p < 0.01, *p < 0.05.