Research Paper Volume 12, Issue 12 pp 11732—11753
Chemerin facilitates intervertebral disc degeneration via TLR4 and CMKLR1 and activation of NF-kB signaling pathway
Figure 6. TLR4, and CMKLR1 knockdown attenuates the chemerin-induced senescence in human NPCs. (A) The expression levels of p-p53, p53, p21, and p16 were evaluated by western blotting. (B–D) Quantification of p-p53, p53, p21, and p16 immunoblots. (E, G) SA-β gal staining assay was performed in NPCs as treated above. (F) EdU microplate assay was performed in NPCs. Data are represented as mean ± SEM of three independent experiments, each done in triplicate. Significant differences between groups are indicated as **p < 0.01, *p < 0.05.