Research Paper Volume 12, Issue 12 pp 11781—11793

Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions

Figure 1. HSD increases cerebral microvascular permeability. (A) Representative images of the mice cerebral cortical microvessels and dye leakage at 10 min after injection of Rhodamine B isothiocyanate-dextran (n=3 per group). (B) Evans blue leakage analyzed in rats fed with selected diets for 180 days (n=3 per group). (C) ZO-1 (green) co-stained with CD31 (red), a microvascular endothelia marker, and DAPI (blue; n=6 per group) in the rats brain slices. (D, E) Expression of Occludin, Claudin-5 and ZO-1 in the brain tissues from rats analyzed by immunoblotting. (F) Systolic blood pressure (SBP) of rats fed with NSD or HSD for 180 days. *P<0.05, **P<0.01, compared with NSD; at least three separate experiments were conducted, means ± SD.