Figure 1. HSD increases cerebral microvascular permeability. (A) Representative images of the mice cerebral cortical microvessels and dye leakage at 10 min after injection of Rhodamine B isothiocyanate-dextran (n=3 per group). (B) Evans blue leakage analyzed in rats fed with selected diets for 180 days (n=3 per group). (C) ZO-1 (green) co-stained with CD31 (red), a microvascular endothelia marker, and DAPI (blue; n=6 per group) in the rats brain slices. (D, E) Expression of Occludin, Claudin-5 and ZO-1 in the brain tissues from rats analyzed by immunoblotting. (F) Systolic blood pressure (SBP) of rats fed with NSD or HSD for 180 days. *P<0.05, **P<0.01, compared with NSD; at least three separate experiments were conducted, means ± SD.