Increased cerebral microvascular permeability in VEGFhi/+ mice. (A) Schematic diagram of VEGFhi/+ mice generation. (B) Immunoblotting showing VEGF, Occludin, Claudin-5 and ZO-1expression in VEGFhi/+ and wildtype mice. (C) Cerebral cortical micro-vessel and dye leakage at 5 and 10 min after injection of Rhodamine B isothiocyanate-dextran; n=5, *P<0.05, **P<0.01, compared with NSD. At least three separate experiments were conducted; means ± SD.