Figure 7. Blocking VEGF attenuates disruption of tight junctions induced by HSD. (A) Representative images showing double immunofluorescence staining of ZO-1 in green and DAPI in the brain specimens of rats. (B, C) Expression of Occludin, Claudin-5 and ZO-1 determined by western blotting; n=5, *P<0.05, **P<0.01, compared with NSD. At least three separate experiments were conducted; means ± SD.