Figure 5. Effect of GALNT6 mediated-mucin-type O-glycosylation of secretory proteins on breast cancer cell migration. (A) VVA-lectin pull-down assay in MDA-MB-231/NC and MDA-MB-231/KD-T6 and detected by western blot at different exposure time points. (B) The expression of FN and MUC1 in MDA-MB-231/NC and MDA-MB-231/KD-T6 was detected by western blot. β-actin was used as internal control. (C) The effect of the supernatant of MDA-MB-231 on migratory ability of MDA-MB-468 cells was detected by transwell assay.