Research Paper Volume 12, Issue 12 pp 11794—11811

GALNT6 promotes breast cancer metastasis by increasing mucin-type O-glycosylation of α2M

Figure 7. Effect of the GALNT6-α2M axis on the activation of PI3K/Akt signaling. (A) Western blot analysis of phosphorylation and baseline protein expression of Akt and ERK1/2 in MDA-MB-468/NC and MDA-MB-468/OE-GALNT6 cells. (B) Western blot analysis of phosphorylation and baseline protein expression of Akt and ERK1/2 in MDA-MB-231/NC and MDA-MB-231/KD-GALNT6 cells. (C) Western blot analysis of phosphorylation and baseline protein expression of Akt in MDA-MB-468 cells transfected with GALNT6 and/or α2M. (D) Western blot analysis of phosphorylation and baseline protein expression of Akt in MDA-MB-468 cells transfected with siRNA targeting GALNT6 and/or α2M. β-actin was used as internal control.