Research Paper Volume 12, Issue 12 pp 11812—11834

FAM83H and SCRIB stabilize β-catenin and stimulate progression of gastric carcinoma

Figure 4. The effect of knock-down or overexpression of FAM83H on the proliferation and invasiveness of gastric cancer cells. (A) The effect of knock-down or overexpression of FAM83H on proliferation were evaluated with an MTT assay, cell counting, and a colony-forming assay in MKN-45 and NCI-N87 cells. The MTT assay was performed after seeding 3,000 cells per well of a 96-well plate. Cell counting was performed after seeding 3,000 cells per well of a 6-well plate. The colony-forming assay was performed by plating 1,000 cells per well in a 6-well plate for ten days. The knock-down or overexpression of FAM83H was assessed via western blot for FAM83H. (B) The migration assay was performed by seeding 1x105 MKN-45 or 1x105 NCI-N87 cells in the upper chamber for 48 hours. (C) The invasion assay was performed by seeding 2x105 MKN-45 or 2x105 NCI-N87 cells in the upper chamber with Matrigel for 48 hours. The chambers were stained with DIFF-Quik staining solution, and the number of migrated or invaded cells were counted in five x200 microscopic fields in each well. *; P < 0.05, **; P < 0.001.