Research Paper Volume 12, Issue 12 pp 12002—12018

Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway

Figure 3. Plasma ED-EVs activate YAP nuclear translocation and promote DSF proliferation. (A) CCK-8 detected DSF viability after ED-EVs or S-NC treatment. (B) EdU assay measured DSF proliferation after ED-EVs or S-NC treatment. (C) Transwell assay measured fibroblast migration after ED-EVs or S-NC treatment. (DF) Western blot analysis measured YAP phosphorylation in DSF, and YAP and CTGF expression in nucleus. Compared with the blank group, *p < 0.05, **p < 0.01, ***p < 0.001. Data in panels (C, E and F) were analyzed by one-way ANOVA, and data in panel D were analyzed by two-way ANOVA, followed by Tukey's multiple comparisons test. Repetitions = 3.