Research Paper Volume 12, Issue 12 pp 12002—12018

Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway

Figure 4. Inhibition of YAP can reverse the effect of plasma ED-EVs on DSF proliferation. (A, B) YAP expression in different transfected DSF was detected by RT-qPCR and western blot analysis. (C) CCK-8 detected DSF viability after different S-NC treatment. (D, E) Transwell assay measured DSF migration after different treatment. (F) Representative images of EdU results in different transfected DSF. Compared with the blank group, *p < 0.05, **p < 0.01, ***p < 0.001; compared to the ED-EVs group, ###p < 0.001; compared to the NC group, &&& p < 0.001. Data in panels (A, B and D) were analyzed by one-way ANOVA, and data in panel C were analyzed by two-way ANOVA, followed by Tukey's multiple comparisons test. Repetitions = 3.