Figure 7. Plasma ED-EVs inhibit premature senescence of skin fibroblasts in diabetic mice through the PI3K/Akt/mTOR pathway and YAP nuclear translocation. (A, B) Western blot analysis detected levels of PI3K/Akt/mTOR pathway-and senescence-related proteins, YAP phosphorylation in fibroblasts, and YAP and CTGF expression in nucleus. Compared with the blank group, ***p < 0.001. Histone H3 was used as the internal parameter of YAP expression in nucleus and β-actin as the internal parameter of cytoplasmic proteins. Data in panel B (i, ii, iii) were analyzed by two-way ANOVA, and data in panel B (iv) were analyzed by one-way ANOVA, followed by Tukey's multiple comparisons test. n = 5.