Figure 1. DLX6-AS1 was up-regulated in BC tissues and cell lines. (A) The DLX6-AS1 expression in 60 cases of BC tissues and adjacent normal tissues were determined by qRT-PCR. (B) The relationship of 5 year survival rate of the BC patients with high (n=27) or low (n=33) DLX6-AS1 expression were analyzed by Kaplan-Meier’s method and the log-rank test. (C) The expression of DLX6-AS1 in BC tissues and normal tissues was determined by ISH assay. (D) The mRNA expression level of DLX6-AS1 in a human bladder epithelium immortalized cell line (SV-HUC-1) and five BC cell lines (T24, RT4, 5637, J82, and SW780) was determined by qRT-PCR. The DLX6-AS1 was highly expressed in BC cells. Data were expressed as the mean ± SD. ***P < 0.001 vs SV-HUC-1.