Research Paper Volume 12, Issue 16 pp 16021—16034

Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway

Figure 6. Effect of DLX6-AS1 and miR-195-5p on proliferation, migration, and invasion of BC cells and phosphorylation of VEGFA, Ras, MEK1/2, ERK1/2. (AC) BC cells were transfected with siNC, siNC + si-DLX6-AS1, si-DLX6-AS1 + NC inhibitor, or si-DLX6-AS1 + miR-195-5p inhibitor. DLX6-AS1 expression (A), miR-miR-195-5p expression (B), and VEGFA mRNA expression (C) were examined by qRT-PCR assay. (D) Cell proliferation of BC cells was identified by CCK8 kit. (E) Cell apoptosis was examined by TUNEL assay. (F) Cell migration was detected by wound healing assay. (G) Cell migration was identified by transwell assay. (H) The effect of sh-DLX6-AS1 and miR-195-5p inhibitor on protein expression and phosphorylation levels of VEGFA and Ras/ERK signaling molecules was evaluated by western blot. Data were expressed as the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.