Research Paper Volume 12, Issue 12 pp 12074—12085

Agonism of GPR120 prevented IL-1β-induced reduction of extracellular matrix through SOX-9

Figure 8. Knockdown of GPR120 abolished the protective effects of GW9508 on the expression of SOX-9. Cells were transfected with non-specific siRNA (NS) or GPR120 siRNA (siGPR120) for 24 h, followed by stimulation with or without GW9508 (50 μM) for 24 h. (A) Western blot analysis revealed the successful knockdown of GPR120. (B) SOX-9 mRNA; (C) SOX-9 protein (^^^, P<0.0001 vs. control group; ***, P< 0.0001 vs. IL-1β treatment group, ###, P<0.0001 vs. IL-1β+ GW9508, n=4-5).