Figure 10. Effects of introduction of DDR1b on Raf/MEK/ERK and Akt protein levels in PC3 Prostate cancer cells. Western blot analysis was performed to determine the levels DDR1 and key members of the RAF/MEK/ERK pathway and Akt in response to introduction of DDR1b. Cells were cultured in the presence and absence of 0.1 gelatin which in some circumstances activates DDR1. The levels of DDR1b were determined upon analysis with an HA-tag antibody as the DDR1b encoding retrovirus has a HA-tag. Levels of β-actin were determined as a protein loading control These experiments were repeated twice, and similar results were observed. The fold values shown in white numbers and letters are presented as averages of 3 densitometric readings. ND = not detected.