Research Paper Volume 12, Issue 11 pp 10194—10210

Influences of TP53 and the anti-aging DDR1 receptor in controlling Raf/MEK/ERK and PI3K/Akt expression and chemotherapeutic drug sensitivity in prostate cancer cell lines

Figure 2. RT-PCR analysis of NGAL, AR, TWIST1 and GAPDH mRNAs in prostate cancer cell lines containing or lacking functional TP53. RT-PCR analysis was performed on 22Rv-1, 22Rv-1+DN-TP53, LNCaP, LNCaP + DN-TP53, DU145, DU145 + WT-TP53, PC3 and PC3 + WT-TP53 cells with oligonucleotides specific for NGAL, AR, TWIST1 and GAPDH. These genes are differentially expressed in the four prostate cancer cell lines.