Figure 9. Effects of introduction of DDR1b on Raf/MEK/ERK and Akt protein levels in DU145 prostate cancer cells. Western blot analysis was performed to determine the levels DDR1 and key members of the Raf/MEK/ERK pathway and Akt and p27Kip-1 in response to introduction of DDR1b. Cells were cultured in the presence and absence of 0.1 gelatin. The levels of DDR1b were determined upon analysis with an HA-tag antibody as the DDR1b encoding retrovirus has a HA-tag. Levels of βactin were determined as a protein loading control These experiments were repeated twice, and similar results were observed. The fold values shown in white numbers and letters are presented as averages of 3 densitometric readings. ND = not detected.