Effect of miR-126 on ESCC cell proliferation. (A, B) miR-126 mRNA levels in TE13 and Eca109 cells treated with miR-126 or NC inhibitors detected using qPCR. (C, D) Soft agar CFA of TE13 and Eca109 cells treated with miR-126 or inhibitors. Lower panel indicates the colony number. (E, F) TE13 and Eca109 cell proliferation rate at 1, 2, and 3 d after transfection. Results are displayed as the average ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.