Figure 5. miR-126 depletion leads to autophagy. (A, B) TE13 and Eca109 cells were plated onto 24-well plates and treated with GFP-LC3 plus either the miR-126 or NC-inhibitor for 36 h before harvesting. GFP-LC3B was assessed using IFA (magnification, × 400). (C, D) WB analysis of LC3B and p62 protein expression in cells after miR-126 silencing. (E, F) qPCR analysis of p62 mRNA expression after miR-126 silencing in TE13 and Eca109 cells. Results are displayed as the average ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.