Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 12 pp 12107—12118
MiR-126 promotes esophageal squamous cell carcinoma via inhibition of apoptosis and autophagy
Figure 6. STAT3 is a direct target of miR-126. WB (A) and qPCR (B) were used to measure STAT3 expression in ESCC cells. (C) Graphical illustration of the conservative miR-126 binding motif in the STAT3 3’-UTR. (D) DLRA with luciferase reporter constructs of WT or MU STAT3 3’-UTR following transfection with the miR-126 mimic. Luciferase activity was standardized to β-galactosidase. Treatment with the miR-126 mimic dramatically reduced the relative luciferase activity in the WT 3’-UTR. WB (E, F) and qPCR (G, H) were used to measure STAT3 protein and mRNA expression following transfection with miR-126 or NC inhibitors. Results are displayed as the average ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.