Figure 8. STAT3 silencing suppresses the apoptosis and autophagy in ESCC cells caused by miR-126 silencing. ESCC cells were treated with an miR-126 inhibitor plus shRNA-STAT3 or shRNA-NC. (A, B) STAT3 knock-down eliminated the increase in apoptosis caused by miR-126 inhibition. FC with annexin V-FITC and PI staining was used to assess early apoptosis in TE13 and Eca109 cells at 36 h post transfection. (C, D) WB was used to examine LC3B and p62 protein levels in cells after transfection. (E, F) qPCR was used to measure p62 mRNA expression in TE13 and Eca109 cells after different treatments. Results are displayed as the average ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.