Research Paper Volume 12, Issue 12 pp 12160—12174

ROCK1 knockdown inhibits non-small-cell lung cancer progression by activating the LATS2-JNK signaling pathway

Figure 2. ROCK1 knockdown decreases cell migration and proliferation. (A) A CCK-8 assay was used to quantify proliferation in A549 cells transfected with siRNA against ROCK1 (si/ROCK1) or control siRNA (si/Ctrl). (B, C) A qPCR assay was used to analyze Cyclin-D and Cyclin-E transcription. (D) Transwell assay for A549 cells. Numbers of migrated cells were quantified after si/ROCK1 or si/Ctrl transfection. (E, F) A qPCR assay was used to analyze CXCR-4 and CXCCR-7 transcription. *p<0.05.