Figure 3. The LATS2-JNK pathway is activated after ROCK1 knockdown. (A) A qPCR assay was used to analyze LATS2 transcription. (B) Western blots were used to detect LATS2 protein levels in response to si/ROCK1 transfection. (C) An ELISA was used to measure JNK activity in response to siRNA-mediated ROCK1 knockdown. (D) A qPCR assay was used to analyze JNK transcription after si/ROCK1 transfection. *p<0.05.