Research Paper Volume 12, Issue 12 pp 12160—12174

ROCK1 knockdown inhibits non-small-cell lung cancer progression by activating the LATS2-JNK signaling pathway

Figure 5. ROCK1 deficiency promotes mitochondrial apoptosis by activating the LATS2-JNK pathway. (A) ATP production was measured in A549 cells after transfection of siRNA against LATS2 (si/LATS2) and SP600125 administration that inhibited LATS2 upregulation and JNK activation, respectively. (B, C) qPCR was used to analyze COX-1 and COX-2 transcription. (D, E) Immunofluorescence was used to measure generation of ROS in A549 cells. (FG) A qPCR assay was used to detect changes in Bax and Bad levels in A549 cells. *p<0.05.