Research Paper Volume 12, Issue 12 pp 12160—12174

ROCK1 knockdown inhibits non-small-cell lung cancer progression by activating the LATS2-JNK signaling pathway

Figure 6. The ROCK1-LATS2-JNK pathway affects mitochondrial dynamics and mitochondrial biogenesis in A549 cells. (AC) Immunofluorescence was used to observe mitochondrial morphology in A549 cells. siRNA against LATS2 (si/LATS2) and SP600125 were used inhibit LATS2 upregulation and JNK activation, respectively. Mitochondrial length and number of cells with fragmented mitochondria were recorded. (DF) A qPCR assay was used to analyze Drp1, Fis1, and Mid49 transcription in A549 cells in response to ROCK1 knockdown, LATS2 knockdown, and JNK inhibition. (G) Double immunofluorescence was used to observe alterations in Sirt3 and PGC1α levels; relative immunofluorescence intensities were evaluated. *p<0.05.