Research Paper Volume 12, Issue 14 pp 14080—14091

CircRNA FGFR3 induces epithelial-mesenchymal transition of ovarian cancer by regulating miR-29a-3p/E2F1 axis

Figure 4. The knockdown of E2F1 suppresses circFGFR3-induced OC EMT and progression. (A). shRNA interference was used to modify the E2F1 mRNA expression in OC cells. (B) Knockdown of E2F1 attenuated circFGFR3-induced upregulation of E2F1 expression in OC cells; (C) and (D) CCK8 assays were used to measure OC cell viability and Transwell assays were used to measure OC cell migration. Data are represented as the mean ± SD, n=3, *P<0.05; **P<0.01; ***P<0.005, bar=200 μm; (E) The cellular morphology of OC cells with circFGFR3 overexpression and different expression levels of E2F1 is shown, bar=200 μm; (F and G) Cells expressing high levels of circFGFR3 showed an increase in the epithelial marker E-cadherin, and decreases in the mesenchymal markers vimentin, fibronectin 1 and N-cadherin when E2F1 was knocked down. (H) Representative images of the tumors from each group (n = 3 mice/group). (I) Representative images of the lung to show the metastasis or no metastasis from each group (bar=50nm).