Research Paper Volume 12, Issue 12 pp 11325—11336

CD38 inhibition by apigenin ameliorates mitochondrial oxidative stress through restoration of the intracellular NAD+/NADH ratio and Sirt3 activity in renal tubular cells in diabetic rats

Figure 2. Apigenin downregulates CD38 in diabetic rats. (A) Representative photographs of immunohistochemical staining of CD38 in the tubulointerstitial area (scale bar: 100 μm). (B) Western blotting of CD38 and control β-actin in the renal cortex. (C) Densitometric evaluation of the western blotting shown in panel B (n=6). (D) Quantitative RT-PCR of CD38 mRNA normalized to 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p<0.01 vs. other groups.