Figure 3. Apigenin increases Sirt3 activity in diabetic rats. (A) Western blots of Sirt3, acetylated (ace) (Lys413)-IDH2, IDH2, acetylated (ace) (Lys68)-SOD2, SOD2, and CoxIV from mitochondrial protein extracts obtained from rat renal cortex. (B–D) Densitometric evaluation of Sirt3 to CoxIV (B), Ace (Lys413)-IDH2 to IDH2 (C), and Ace (Lys68)-SOD2 to SOD2 (D) immunoblotting data shown in panel A (n=6). (E) 8-OHdG content in mitochondria (mt) adjusted to mtDNA in the renal cortex (n=6). (F) NAD+/NADH ratio in isolated mitochondrial protein extracts from rat renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p<0.01 vs. other groups, n.s; not significant.