Figure 5. GLSO downregulated the levels of LPS and TLR4 on early skin wound healing. LPS levels in murine serum (A) and skin (B) was examined by the ELISA. The qPCR analysis revealed the levels of TLR4 at the murine wound edge on day 1 (C), day 3 (D) and day 5 (E). (F) Images of immunofluorescence from the model group and GLSO group at different healing time points were taken by immunofluorescent microscope and were compared with the control group under the microscope 200X. Scale bar = 200 μm. The data are indicated as the mean ± SD. ##P and **P <0.01, ###P and ***P <0.001(#P: control vs model, *P: GLSO vs model).