Research Paper Volume 12, Issue 14 pp 14125—14140

The effect of Ganoderma lucidum spore oil in early skin wound healing: interactions of skin microbiota and inflammation

Figure 6. GLSO accelerates the skin wound healing more rapidly than the group with treatment of antibiotics. (A) The culture of skin microbiota on each group after 7 days with the treatment of antibiotics. (B) Gross examination of the wound area on each group. Scale bar =6 mm. (C) H&E staining of the wound tissue sections. (D) Immunohistochemical analysis of cytokeratin 14 under the microscope 100X, scale bar=400 μm. (E) Masson’s Trichrome staining, scale bar=1 mm. Immunohistochemical analysis of Collagen I (F) and Collagen III (G) at the murine skin wound on each group under the microscope 100X, scale bar=0.4 mm. (H) Quantitative analysis of Masson’s Trichrome staining, and immunostaining of cytokeratin 14 (quantitation results of Fig 1E), Collagen I, and Collagen III (n=3 each group). The data are indicated as the mean±SD. *P<0.05,**P <0.01, ***P <0.001(*P: other groups vs GLSO).