Research Paper Volume 12, Issue 14 pp 14141—14156

Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2

Figure 1. AC092171.4 is upregulated in HCC tissues. (A) Quantitative real-time PCR (qRT-PCR) analysis of AC092171.4 expression in HCC (n=369) and normal liver (n=50) tissues from the GEPIA database. As shown, AC092171.4 levels are higher in HCC tissues compared to normal liver tissues (*p<0.05). (B) QRT-PCR analysis of AC092171.4 expression in 70 pairs of HCC and corresponding ANLTs. As shown, AC092171.4 levels are higher in HCC tissues compared to adjacent normal liver tissues (ANLTs; ****p<0.0001). (C) QRT-PCR analysis showing AC092171.4 expression was significantly higher in the Huh7 and LM3 HCC cell lines than PLC/PRF/5 and Hep3B cells. (D) Chromogenic in situ hybridization (CISH) analysis of AC092171.4 expression in 95 pairs of HCC and ANLTs in a representative photograph. The results show that AC092171.4 expression is higher in HCC tissues compared to ANLTs (p<0.001). (E) Kaplan-Meier survival curve analyses of overall survival (OS) and disease-free survival (DFS) in HCC patients with low (n=42) and high (n=53) AC092171.4 expression. (F) Kaplan-Meier survival curve analyses of overall survival (OS) and disease-free survival (DFS) in high and low AC092171.4-expressing HCC patients of the GEPIA dataset (tumor=369, normal=50). * denotes p<0.05.