Research Paper Volume 12, Issue 14 pp 14141—14156

Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2

Figure 4. AC092171.4 knockdown represses in vivo xenograft HCC tumor growth and pulmonary metastases. (A) Balb/c nude mice were subcutaneously injected with sh-NC or sh-AC092171.4 transfected Huh7 cells and tumor from respective groups were shown (n=5). (B, C) Xenograft tumor volume and weights in nude mice subcutaneously injected with sh-NC or sh-AC092171.4 transfected Huh7 cells. (D, E) Immunohistochemical analysis shows percentage of Ki-67-positive stained cells in the sections of xenograft tumors from control and AC092171.4 knockdown nude mice. (F, G) Total numbers of pulmonary metastatic nodules in nude mice injected with control and AC092171.4 knockdown Huh-7 cells through the tail vein. * denotes p<0.05.