Figure 3. Hsa_circ_0000073 accelerated acquisition of MTX resistance and proliferation of MTX-resistant OS cells in vitro. (A) The IC50 of MTX was examined via CCK-8 assay in MTX-resistant MG-63 and U2OS cells. (B) Hsa_circ_0000073 expression was monitored through qRT-PCR assay in MTX-resistant MG-63 and U2OS cells. (C) Hsa_circ_0000073 expression was determined by qRT-PCR in OS patients with good or poor response to MTX. (D, E) After hsa_circ_0000073 overexpression or knockdown, the IC50 of MTX was determined using a CCK-8 assay in MG-63 and U2OS cells. (F) The impact of hsa_circ_0000073 knockdown on cell proliferation was assessed by colony formation assay in MTX-resistant MG-63 and U2OS cells. *P<0.05.