Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 14 pp 14157—14173
Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS
Figure 3. Hsa_circ_0000073 accelerated acquisition of MTX resistance and proliferation of MTX-resistant OS cells in vitro. (A) The IC50 of MTX was examined via CCK-8 assay in MTX-resistant MG-63 and U2OS cells. (B) Hsa_circ_0000073 expression was monitored through qRT-PCR assay in MTX-resistant MG-63 and U2OS cells. (C) Hsa_circ_0000073 expression was determined by qRT-PCR in OS patients with good or poor response to MTX. (D, E) After hsa_circ_0000073 overexpression or knockdown, the IC50 of MTX was determined using a CCK-8 assay in MG-63 and U2OS cells. (F) The impact of hsa_circ_0000073 knockdown on cell proliferation was assessed by colony formation assay in MTX-resistant MG-63 and U2OS cells. *P<0.05.