Research Paper Volume 12, Issue 17 pp 16803—16819

Anchorage independence altered vasculogenic phenotype of melanoma cells through downregulation in aminopeptidase N /syndecan-1/integrin β4 axis

Figure 3. ANPEP expression was downregulated upon cell suspension. (A) The expression level of ANPEP in adherent, suspended, and re-attached melanoma cells as examined by qPCR and flow cytometry. Downregulation of ANPEP at the surface of suspended or re-attached melanoma cells was observed. Data were mean ±S.D. (n=3); **, p < 0.01. (B) Expression of integrin isoforms in adherent and suspended melanoma cells as examined by qPCR. Data were mean ±S.D. (n=3); *, p < 0.05; **, p < 0.01. (C) Integrin α6, β2, and β4 protein expression upon cell suspension as examined by western blot.