Figure 3. TINCR is a molecular sponge to interact with miR-31 in CRC. (A) The binding (site) between TINCR and miR-31 was predicted and shown by StarBase. (B, C) RT-qPCR for miR-31 in as-miR-31-transfected Caco-2 cells vs scrambled-transfected Caco-2 cells (B), and in miR-31-transfected SW480 cells vs scrambled-transfected SW480 cells (C). (D, E) A luciferase reporter assay was done to examine whether TINCR could interact with miR-31 in SW480 cells by co-transfection of miR-31 with TINCR-wt or TINCR-mut (D), and to examine whether TINCR could interact with miR-31 in Caco-2 cells by co-transfection of as-miR-31 with TINCR-wt or TINCR-mut (E). N=5. *p<0.05. NS: non-significant.