Figure 2. DLS and NTA analysis. (A) EV size characterization by DLS and NTA. No variation is observed for the EV size in older and younger women. A small difference in the average diameters of EVs is generally found between DLS (where the hydrodynamic radius is measured) and SEM and NTA (that measure the actual radius). (B) Dynamic Light Scattering (DLS) measurements and Nanoparticle tracking analysis (NTA) of small EVs from FFs of older and younger women. Scattered light Intensity, expressed as Kilocounts-per-second (Kcps), and nanoparticle concentration for FFs are shown as red and blue bars respectively. (C) Significant positive linear relationship between DLS and NTA measurements on FF samples. Pearson’s coefficient of correlation (r), R2, and P-value are reported. (D) DLS and (E) NTA show a significantly higher concentration of exosomes in FFs from older women. Kcps and concentration differences related to small EVs of younger and older women are reported as Box plots with Whiskers. The ratio of small EV concentrations ® between younger and older women for DLS and NTA is shown. ** P-values<0.01; * P-value <0.05.