Figure 4. C5aR deficiency or C5aR signaling blockade inhibits the downregualtion of p21 expression in tumor tissues of mice. Mice were received 4T-1 cells transplantation for tumor development and sacrificed in the indicated time. (C5aRa treatment group: WT mice were injected with C5aRa 30 min prior to 4T-1 cells transplantation. Saline was used as the control of C5aRa). The tumors tissues were collected. The expression of p21 and p-p21 was measured by western blotting (A) and immunohistochemistry (B, C). (D) The mRNA levels of p21 were measured by quantitative RT-PCR. (n=5-6/group). *P < 0.05, ***P < 0.001. GAPDH was used as the loading control. Magnification: 200×.