Figure 6. Reduced p38 phosphorylation in BC tumoral tissues in mice with C5aR deficiency or C5aR signaling blockade. (A) The phosphorylation levels of p38 in human BC cell line MCF-7 cells after C5aRa treatment (10 nM) for indicated times. (B, C) After transplantation with murine BC cell line 4T-1 cells, mice were sacrificed in the indicated times and tumor tissues were collected (C5aRa treatment group: WT mice was injected with C5aRa 30 min prior to 4T-1 cells transplantation. Saline was used as the control of C5aRa). P-p38/p38 expression levels in tumor tissue were determined by western blotting. GAPDH was used as the loading control. The data are representative of three independent experiments.