Research Paper Volume 12, Issue 14 pp 14285—14299

C5aR deficiency attenuates the breast cancer development via the p38/p21 axis

Figure 6. Reduced p38 phosphorylation in BC tumoral tissues in mice with C5aR deficiency or C5aR signaling blockade. (A) The phosphorylation levels of p38 in human BC cell line MCF-7 cells after C5aRa treatment (10 nM) for indicated times. (B, C) After transplantation with murine BC cell line 4T-1 cells, mice were sacrificed in the indicated times and tumor tissues were collected (C5aRa treatment group: WT mice was injected with C5aRa 30 min prior to 4T-1 cells transplantation. Saline was used as the control of C5aRa). P-p38/p38 expression levels in tumor tissue were determined by western blotting. GAPDH was used as the loading control. The data are representative of three independent experiments.